• Modeling Mechanism：

  A high-cholesterol diet can increase serum cholesterol, induce lipid deposition in the aorta, and activate the inflammatory response (increased aortic cytokines), making it suitable for research on the inflammatory mechanisms of hyperlipidemia.

• Related Products：

  Cholesterol (T0760)

• Modeling Method：

  Experimental Subject：

  Guinea pigs, Hartley strain, Male, 8 weeks old, Body weight 300–350 g

  Dosage and Administration Route：

  ① Core modelling:
  - High-fat diet (0.5% cholesterol+5% butter);
  ② Control treatment: Control group administered cholesterol-free standard diet, all other conditions identical;

  Dosing Frequency and Duration Model：

  Ad libitum feeding, 10 weeks duration

• Validation：

  1. Biochemical indicators: - Serum cholesterol: ≥5 mmol/L after 10 weeks of modeling (compared to 1.8 mmol/L in the control group); - Inflammatory factors: Serum TNF-α and IL-6 levels were significantly elevated; 2. Pathological indicators: - Aortic lipid deposition: Oil Red O staining showed intimal lipid aggregation, without obvious fibrous plaques; 3. Molecular indicators: - Upregulated expression of aortic inflammatory genes (VCAM-1, ICAM-1).

*Precautions：

*References：Xiangdong L,et,al. Animal models for the atherosclerosis research: a review. Protein Cell. 2011 Mar;2(3):189-201.