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> Cattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Cattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

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Référence NB-66-38837-96T

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Cattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
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ELK0100
Size： ：
48 T
96 T
96 T*5
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Overview

Product name: Cattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Reactivity: Cattle
Alternative Names: CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type: Sandwich
Sensitivity: 26.1 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Uniprot ID: P13789
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.166 2.057
2500.00 1.697 1.588
1250.00 1.167 1.058
625.00 0.899 0.790
312.50 0.536 0.427
156.25 0.461 0.352
78.13 0.114 0.005
0.00 0.109 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 79-97% 88%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-94% 79-96% 85-99%
EDTA plasma(n=5) 88-101% 86-92% 83-98% 96-103%
Heparin plasma(n=5) 85-94% 87-103% 81-95% 82-90%