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> Anti-Androgen receptor Antibody (5S754)

Anti-Androgen receptor Antibody (5S754)

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Référence NB-64-152624-100uL

Conditionnement : 100uL

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Anti-Androgen receptor Antibody (5S754)

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Catalog No. TMAB-02545 Copy Product Info
Anti-Androgen receptor Antibody (5S754) is a Rabbit antibody targeting Androgen receptor. Anti-Androgen receptor Antibody (5S754) can be used in WB,IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Androgen receptor Antibody (5S754) is a Rabbit antibody targeting Androgen receptor. Anti-Androgen receptor Antibody (5S754) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
5S754
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Androgen receptor Monoclonal Antibody, Unconjugated (TMAB-02545) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Androgen receptor Monoclonal Antibody, Unconjugated (TMAB-02545) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Androgen receptor Monoclonal Antibody, Unconjugated (TMAB-02545) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Androgen receptor Monoclonal Antibody, Unconjugated (TMAB-02545) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Androgen receptor Monoclonal Antibody, Unconjugated (TMAB-02545) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with Androgen receptor monoclonal antibody, unconjugated (TMAB-02545) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Predominantly cytoplasmic in unliganded form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unliganded form in the presence of GNB2L1.
Tissue SpecificityIsoform 2 is mainly expressed in heart and skeletal muscle.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Antigen Details
Immunogen
A synthesized peptide: human Androgen receptor
Antigen Species
Human
Gene Name
AR
Gene ID
367
Protein Name
Androgen receptor
Uniprot ID
P10275
Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3.
Chemical Properties
Molecular WeightTheoretical: 99 kDa. Actual: 105 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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