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Prolactin Protein, Mouse, Recombinant (His)

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Référence NB-64-57532-10ug

Conditionnement : 10ug

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Prolactin Protein, Mouse, Recombinant (His)

(Synonyms: prolactin, Prl1a1, AV290867) Copy Product Info

Synonyms: prolactin, Prl1a1, AV290867

Catalog No. TMPY-02424 Copy Product Info

Prolactin Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 23.8 kDa and the accession number is Q9CPQ2.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:≥ 92 % as determined by SDS-PAGE.≥ 90 % as determined by SEC-HPLC.

Color:White

Product Information

Bioactivity

Bioactivity	Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is typically 50-500 ng/mL.
Description	Prolactin Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 23.8 kDa and the accession number is Q9CPQ2.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q9CPQ2
Construction	A DNA sequence encoding the mouse PRL (NP_035294.2) (Leu 32-Cys 228) was expressed，with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus. Predicted N terminal: Met
Protein Purity	≥ 92 % as determined by SDS-PAGE.≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	prolactin, Prl1a1, AV290867
Research Background	Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.

Chemical Properties

Molecular Weight	23.8 kDa (predicted); 26 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

AV-290867AV 290867

Related Tags: Prolactin Protein, Mouse, Recombinant (His) chemical structure | Prolactin Protein, Mouse, Recombinant (His) molecular weight