General Information
| Short Description | GTPase HRAS Positive Control for STJ502036 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:500 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Specificity | This is positive control is recommended for use in combination with GTPase HRAS antibody STJ502036. |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling. |
| Function | Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Peptide Name | Gtpase HrasH-Ras-1Ha-RasTransforming Protein P21C-H-RasP21ras Cleaved Into - Gtpase Hras - N-Terminally Processed |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusGolgi Apparatus MembraneThe Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound FormShuttles Between The Plasma Membrane And The Golgi ApparatusIsoform 2: NucleusCytoplasmPerinuclear RegionColocalizes With Rack1 To The Perinuclear Region |
| Alternative Peptide Names | Gtpase Hras proteinH-Ras-1 proteinHa-Ras proteinTransforming Protein P21 proteinC-H-Ras proteinP21ras Cleaved Into - Gtpase Hras - N-Terminally Processed proteinHRAS proteinHRAS1 protein |
Information sourced from Uniprot.org