Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Enoyl-ACP Reductase Protein, E. coli, Recombinant (His)

Enoyl-ACP Reductase Protein, E. coli, Recombinant (His)

Imprimer Imprimer
Identifiez-vous

Référence NB-64-56944-20ug

Conditionnement : 20ug

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Remove All

Enoyl-ACP Reductase Protein, E. coli, Recombinant (His)

(Synonyms: qmeA, JW1281, gts, envM, ECK1283, b1288) Copy Product Info

Synonyms: qmeA, JW1281, gts, envM, ECK1283, b1288

Catalog No. TMPY-01812 Copy Product Info
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to theshort-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to theshort-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
Species
E. coli
Expression System
E. coli
TagN-His
Accession NumberNP_287908.1
ConstructionA DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 98 % as determined by SDS-PAGE
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris 0.5M NaCl, 10% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsqmeA, JW1281, gts, envM, ECK1283, b1288
Research Background
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to theshort-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
Chemical Properties
Molecular Weight29.5 kDa (predicted); 33 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Dose Conversion

Keywords

JW-1281JW 1281ECK-1283ECK 1283
Related Tags: Enoyl-ACP Reductase Protein, E. coli, Recombinant (His) chemical structure | Enoyl-ACP Reductase Protein, E. coli, Recombinant (His) molecular weight