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Cholic Acid [81-25-4]

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Référence NB-64-18522-1g

Conditionnement : 1g

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Cholic Acid

(Synonyms: Colalin, Cholate, Cholanic acid) Copy Product Info

Synonyms: Colalin, Cholate, Cholanic acid

Catalog No. T2963 Copy Product Info

Purity: 99.92%

Cholic Acid (Cholate) is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. Cholic acid facilitates fat absorption and cholesterol excretion.

Cas No. 81-25-4

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.92%

Color:White

Product Introduction

Cholic Acid AI Summary

Cholic Acid exhibits a wide range of bioactivities. It shows a binding affinity with a binding constant of 900.0 Kb M^-1 toward cyclodextrin-conjugated peptides (with pyrene) at 25 degrees Celsius, also demonstrating a change in sensitivity of 0.008 towards the same peptide under identical conditions. It inhibits Na+-dependent [3H]taurocholate uptake in rabbit ileal brush border membrane vesicles with an IC25 of 40.0 µM, IC50 of 83,000.0 nM, and IC75 of 152.0 µM. It also exhibits antibacterial activity against various strains of Escherichia coli, with MIC values ranging from 4.0 to 16,000.0 µg/mL. Furthermore, Cholic Acid acts as an agonist at human TGR5, with an EC50 of 13,600.0 nM and an efficacy of 101.0% relative to lithocholic acid. It also modulates human MRP2-mediated estradiol-17-beta-glucuronide transport in Sf9 cells with an activity level of 112.0% relative to control. It demonstrates antifungal activity with MIC values >128.0 µg/mL for multiple fungi and antibacterial activity against Staphylococcus aureus with a MIC >128.0 µg/mL. The compound shows both agonist and antagonist activities at VDR-LBD and has significant bioactivity against SARS-CoV-2 induced cytotoxicity in several cell models, demonstrating inhibition rates ranging from -0.09% to 6.09% at 10 µM. Additionally, it inhibits HDAC6 and PDE3A and exerts cytotoxic effects on various human cancer cell lines with IC50 values greater than 200,000.0 nM. Cholic Acid is characterized by high water solubility, moderate binding affinity to albumin, and complex effects on cell viability and protein stability across different domains..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Cholic Acid (Cholate) is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. Cholic acid facilitates fat absorption and cholesterol excretion.
Synonyms	Colalin, Cholate, Cholanic acid

Chemical Properties

Molecular Weight	408.57
Formula	C₂₄H₄₀O₅
Cas No.	81-25-4
Smiles	O[C@H]1[C@]2([C@]3([C@@](C)([C@@]([C@@H](CCC(O)=O)C)(CC3)00)[C@@H](O)C[C@@]2([C@]4(C)[C@](C1)(C[C@H](O)CC4)00)00)00)00
Relative Density.	1.128 g/cm3.

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 175 mg/mL (428.32 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.9 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4476 mL	12.2378 mL	24.4756 mL	122.3781 mL
5 mM	0.4895 mL	2.4476 mL	4.8951 mL	24.4756 mL
10 mM	0.2448 mL	1.2238 mL	2.4476 mL	12.2378 mL
20 mM	0.1224 mL	0.6119 mL	1.2238 mL	6.1189 mL
50 mM	0.0490 mL	0.2448 mL	0.4895 mL	2.4476 mL
100 mM	0.0245 mL	0.1224 mL	0.2448 mL	1.2238 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

taurineInhibitorinhibitHepG2glycineEndogenousMetaboliteEndogenous MetaboliteCholic Acidcholesterol excretion

Related Tags: Cholic Acid chemical structure | Cholic Acid formula | Cholic Acid molecular weight