General Information
| Short Description | Goat polyclonal Clathrin-H Chain (50aa to N-Term) antibody for use in WB and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet included with |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:1000IF 1:25-1:250Imbastari F PhD Thesis Humboldt-Universität zu Berlin Germany 2020Caì Y Postnikova EN Bernbaum JG et al. J Virol. 2015 Jan 1:89 (1:844-56. PMID: 25355889 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Accession Number | ENSG00000141367 |
| Immunogen | Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli. |
| Immunogen Region | 50aa to N-Term |
| Immunogen Sequence | MAQILPIRFQEHLQLQNLGI AQILPIRFQEHLQLQNLGIN QILPIRFQEHLQLQNLGINP |
| Specificity | Detects endogenous levels of total CLTC by Western blot in the whole cell lysates (MDCK, At-T20, COS-7). |
Additional Info
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating. |
| Protein Name | Clathrin Heavy Chain 1Clathrin Heavy Chain On Chromosome 17Clh-17 |
| Database Links | |
| Cellular Localisation | Cytoplasmic Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideMembraneCoated PitMelanosomeCytoplasmCytoskeletonSpindleCytoplasmic Face Of Coated Pits And VesiclesIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Complex With Tacc3 And Ckap5 (Forming The Tacc3/Ch-Tog/Clathrin Complex) Localized To Inter-Microtubule Bridges In Mitotic Spindles |
| Alternative Antibody Names | Anti-Clathrin Heavy Chain 1 antibodyAnti-Clathrin Heavy Chain On Chromosome 17 antibodyAnti-Clh-17 antibodyAnti-CLTC antibodyAnti-CLH17 antibodyAnti-CLTCL2 antibodyAnti-KIAA0034 antibody |
Information sourced from Uniprot.org