For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal,
N-Phenylacrylamide [2210-24-4]
Referencia NB-64-45648-2g
embalaje : 2g
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N-Phenylacrylamide
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Catalog No. T9322 Copy Product Info
Purity: 99.99%
N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.
Cas No. 2210-24-4
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.99%
Appearance:solid
Color:White
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Product Information
N-Phenylacrylamide AI Summary
N-Phenylacrylamide is a bioactive inhibitor of human recombinant Transglutaminase 2 (TG2), with an IC50 value of 43000.0 nM based on fluorescent transamidation assay results. It exhibits various pharmacokinetic properties, including a half-life of 4.983 hours in phosphate buffer at pH 7.4 at a concentration of 50 uM in the presence of glutathione. It shows intrinsic clearance rates in human and rat hepatocytes of approximately 30.9 and 32.36 uL/min/(10^6 cells), respectively, indicating moderate metabolism and elimination in liver cells. Additionally, N-Phenylacrylamide inhibits several enzymes—such as West Nile virus NS2B-NS3 protease, Dengue virus serotype 2 NS2B-NS3 protease, Bovine thrombin, human and Escherichia coli MetAP, and wild-type Escherichia coli Mur enzymes—with inhibition rates of less than 15.0%. It also inhibits human FBPase by 17.0% at 500 uM. Furthermore, the compound demonstrates GSH reactivity and forms adducts, evident from RP-HPLC analysis, and shows a pseudo-first-order reaction with GSH at 37°C (K = 13.0 x 10^-3/min) with a half-life of 0.88 hours for GSH reactivity. In terms of thiol reactivity, N-Phenylacrylamide has a pseudo-first-order rate constant of 1.6 x 10^-4/s in a PBS/DMSO buffer environment. When tested against cysteine in PBS buffer using NMR assay, it has a half-life of 2.3 hours and a rate constant of 8.38 x 10^-5/s. Additionally, it shows a half-life of 4.75 hours in GSH reactivity assessment using DNTB and exhibits a Gibbs free energy change of 24.1 kcal/mol at 1 uM concentration within 120 minutes, with a pseudo-first-order rate constant of 3.87 x 10^-3/min and a half-life of 2.983 hours under pH 7.4 phosphate buffer conditions. N-Phenylacrylamide also manifests cytotoxicity against HeLa cells, with an EC50 greater than 100,000.0 nM, further highlighting its potential bioactivity and reactivity in various biological contexts..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay. |
| Targets & IC50 | TGase 2:43 µM |
| Molecular Weight | 147.174 |
| Formula | C9H9NO |
| Cas No. | 2210-24-4 |
| Smiles | C=CC(NC1=CC=CC=C1)=O |
| Relative Density. | 1.31g/cm3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 55.00 mg/mL (373.71 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
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For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal,