General Information
| Short Description | HRP-conjugated chicken polyclonal anti-Goat IgG secondary antibody. For use in most research applications. |
| Applications | WB/ELISA |
| Host | Chicken |
| Reactivity | Goat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | HRP |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000ELISA 1:20000-1:100000 |
| Formulation | PBS, 50% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE. |
| Specificity | Using serum samples detects goat IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Chicken Anti-Goat IgG antibody {HRP} (STJ140211)
This antibody is raised in chicken and is specific to goat IgG. It is suitable for detecting primary antibodies produced in goat, with reduced cross-reactivity to immunoglobulins from other species.
This antibody is conjugated to HRP and has been validated for use in Western blot and ELISA applications.
For Western blot, a dilution of 1:2000 to 1:10000 is recommended. For ELISA, the recommended dilution range is 1:20000 to 1:100000.
The product information notes reduced cross-reactivity to species other than goat, but does not specify which species were tested. Researchers working with samples containing immunoglobulins from multiple species should validate specificity in their own experimental system.
For continuous use, store at 2 to 8 degrees C for one to two days. For extended storage, keep at minus 20 degrees C. Working dilution samples should be discarded if not used within 12 hours.
This antibody is supplied at 2 mg/mL in PBS containing 50% glycerol and 0.05% sodium azide. It has been epitope affinity purified, and the immunogen was full goat IgG with purity greater than 95% by SDS-PAGE.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.