Primary Human Arterial Endothelial Cells (Pooled) (CSC 2A0) are isolated from umbilical arteries of multiple donors.

Cell Initiation

These cells were originated using Neo Biotech Complete Serum-Free Medium , and subsequently grown and passaged in Neo Biotech Complete Medium . They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Neo Biotech Cell Freezing Medium™ . This vial will initiate a Passage 4 cell culture in a 75cm² flask. Each vial contains more than 1 x 10⁶cells.

In addition to cryopreserved vials, these cells also are available in 25cm² and 75cm² proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™

ADD TO CART

The System™

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Passage Reagent Group™

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Standard Tests:

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Cytoplasmic VWF / Factor VIII	> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL	> 95% positive by immunofluorescence

Citations

"A putative angiogenin receptor in angiogenin-responsive human endothelial cells" Hu et al. PNAS, 1997.

Primary Human Arterial Endothelial Cells

Referencia NB-11-0113

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