Notch 1 Protein, Human, Recombinant (His & Avi)
Referencia NB-64-54210-5ug
embalaje : 5ug
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Notch 1 Protein, Human, Recombinant (His & Avi)
(Synonyms: TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik) Copy Product InfoSynonyms: TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik
Catalog No. TMPK-00467 Copy Product Info
Notch 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay. |
| Description | Notch 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P46531 |
| Construction | Ala19-Gln526 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik |
| Research Background | Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Molecular Weight | 56.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
TAN-1TAN 1NOTCH-1NOTCH 1N-1N 1
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Related Tags: Notch 1 Protein, Human, Recombinant (His & Avi) chemical structure | Notch 1 Protein, Human, Recombinant (His & Avi) molecular weight
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