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Synonyms: NADH, disodium salt hydrate, Disodium NADH
| Description | NADH disodium salt hydrate belongs to natural products and is a coenzyme of oxidoreductases with oral activity, serving as an electron donor in processes such as glycolysis, the tricarboxylic acid cycle, and β-oxidation. This compound is widely used in metabolism-related experimental research. |
| In vitro | Methods: In an ethanol system, different concentrations of NADH (0–40 μM) were mixed with DPPH solution, incubated at room temperature for 30 minutes, and absorbance at 517 nm was measured. Results: NADH exhibited concentration-dependent DPPH radical scavenging activity, but with lower potency than gallic acid. [1] Methods: Different concentrations of NADH (0–50 mM) were mixed with Pdots, incubated at room temperature, and fluorescence spectra were measured. Results: Pdots showed high sensitivity and selectivity for NADH. [2] Methods: NADH disodium salt (2 mM) was mixed with R848-N3 (1 mM) and TBR (2 mM), sonicated (1.0 MHz, 2.5 W/cm², 5 min), and analyzed by HPLC. Results: NADH disodium salt successfully mediated the reduction of R848-N3 to R848, confirming its in vitro activity as an electron donor. [3] |
| In vivo | Methods: NADH disodium salt (5 μMol/mouse, single dose) was administered to mice by intraperitoneal injection, and urine was collected to detect the excretion of nicotinamide and its metabolites. Results: NADH disodium salt increased urinary excretion of nicotinamide and its metabolites in mice. [4] Methods: Ethanol-loaded mice were given NADH disodium salt (500 mg/kg, single dose) by gavage to observe alcohol metabolism and liver injury indicators. NADH disodium salt (1000 mg/kg, single dose) was administered to male C57BL/6J mice by intraperitoneal injection, and tissue NAD+ levels were measured. Results: NADH disodium salt promoted alcohol metabolism and prevented and ameliorated early liver injury induced by acute alcohol exposure; NADH disodium salt enhanced tissue NAD+ levels in mice. [5] |
| Synonyms | NADH, disodium salt hydrate, Disodium NADH |
| Molecular Weight | 709.4 |
| Formula | C21H27N7Na2O14P2 |
| Cas No. | 606-68-8 |
| Smiles | [Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O |
| Relative Density. | 1.955 g/cm3 at 20℃ |
| Storage | Store under nitrogen,Store at low temperature,Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 100 mg/mL (140.96 mM), Sonication is recommended.  H2O: 250 mg/mL (352.41 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (4.65 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO/H2O
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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