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> IBV (strain M41) Spike glycoprotein (His)

IBV (strain M41) Spike glycoprotein (His)

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IBV (strain M41) Spike glycoprotein (His)

(Synonyms: Spike glycoprotein, S glycoprotein, Peplomer protein, E2) Copy Product Info

Synonyms: Spike glycoprotein, S glycoprotein, Peplomer protein, E2

Catalog No. TMPH-00148 Copy Product Info
IBV (strain M41) Spike glycoprotein (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 26.8 kDa and the accession number is P12651.
Other Forms of IBV (strain M41) Spike glycoprotein (His):
IBV (strain D274) Spike glycoprotein (His)
IBV (strain D3896) Spike glycoprotein (His)
IBV (strain UK/142/86) Spike glycoprotein (His)
IBV (strain UK/167/84) Spike glycoprotein (His)
IBV (strain UK/123/82) Spike glycoprotein (His)
IBV (strain UK/123/82) Spike glycoprotein (E. coli, His)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:90%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
IBV (strain M41) Spike glycoprotein (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 26.8 kDa and the accession number is P12651.
Species
IBV
Expression System
E. coli
TagC-6xHis
Accession NumberP12651
Amino AcidESNFMYGSYHPSCNFRLETINNGLWFNSLSVSIAYGPLQGGCKQSVFSGRATCCYAYSYGGPSLCKGVYSGELDLNFECGLLVYVTKSGGSRIQTATEPPVITRHNYNNITLNTCVDYNIYGRTGQGFITNVTDSAVSYNYLADAGLAILDTSGSIDIFVVQGEYGLTYYKVNPCEDVNQQFVVSGGKLVGILTSRNETGSQLLENQFYIKITNGTRRFRR
Construction317-537 aa
Protein Purity
> 90% as determined by SDS-PAGE.
IBV (strain M41) Spike glycoprotein (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsSpike glycoprotein, S glycoprotein, Peplomer protein, E2
Research Background
attaches the virion to the host cell membrane by interacting with sialic acids, initiating the infection.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide after S2 cleavage occurring upon virus endocytosis.
Chemical Properties
Molecular Weight26.8 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

S-glycoproteinSE 2
Related Tags: IBV (strain M41) Spike glycoprotein (His) chemical structure | IBV (strain M41) Spike glycoprotein (His) molecular weight