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> Calcineurin A/PPP3CA Protein, Human, Recombinant (His)

Calcineurin A/PPP3CA Protein, Human, Recombinant (His)

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Calcineurin A/PPP3CA Protein, Human, Recombinant (His)

(Synonyms: protein phosphatase 3, catalytic subunit, α isozyme, protein phosphatase 3, catalytic subunit, alpha isozyme, PPP2B, CNA1, CCN1, CALNA1, CALNA, CALN) Copy Product Info

Synonyms: protein phosphatase 3, catalytic subunit, α isozyme, protein phosphatase 3, catalytic subunit, alpha isozyme, PPP2B, CNA1, CCN1, CALNA1, CALNA, CALN

Catalog No. TMPY-02963 Copy Product Info
PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.
Other Forms of Calcineurin A/PPP3CA Protein, Human, Recombinant (His):
CYR61/CCN1 Protein, Human, Recombinant (hFc)TMPJ-00658
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For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9 nM.
Description
PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.
Species
Human
Expression System
Baculovirus Insect Cells
TagN-His
Accession NumberQ08209-1
ConstructionA DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus. Predicted N terminal: His
Protein Purity
> 94 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsprotein phosphatase 3, catalytic subunit, α isozyme, protein phosphatase 3, catalytic subunit, alpha isozyme, PPP2B, CNA1, CCN1, CALNA1, CALNA, CALN
Research Background
PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.
Chemical Properties
Molecular Weight60.8 kDa (predicted); 60 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

CNA-1CNA 1CCN-1CCN 1CALNA-1CALNA 1
Related Tags: Calcineurin A/PPP3CA Protein, Human, Recombinant (His) chemical structure | Calcineurin A/PPP3CA Protein, Human, Recombinant (His) molecular weight