General Information
| Short Description | Goat polyclonal Tubulin, alpha 4a (400aa C-Term) antibody for use in WB and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet included with |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:2000IF 1:50-1:200Ferreira JV Soares AR Ramalho J et al. Sci Adv 2022 Mar PMID: 35333565Saviano A De Vita S Chini MG et al. Biomolecules 2022 Jan PMID: 35053247Ferreira JV Rosa Soares A Ramalho JS et al. PLoS One 2019 Oc |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Accession Number | ENSG00000127824 |
| Immunogen | Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli. |
| Immunogen Region | 400aa C-Term |
| Immunogen Sequence | MYAKRAFVHWYVGEGMEEGE YAKRAFVHWYVGEGMEEGEF AKRAFVHWYVGEGMEEGEFS |
| Specificity | This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat) , rat (TR-iBRB) , mouse (AtT-20, Hepa) , canine (D17) and monkey (COS-7) whole cell lysates. |
Additional Info
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Although this tubulin does not encode a C-terminal tyrosine, a C-terminal tyrosine can be added post-translationally by the tubulin tyrosine ligase (TTL). It can then undergo a detyrosination cycle by the tubulin tyrosine carboxypeptidase (MATCAP1/KIAA0895L). |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Alpha-4a ChainAlpha-Tubulin 1Testis-Specific Alpha-TubulinTubulin H2-AlphaTubulin Alpha-1 Chain |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Tubulin Alpha-4a Chain antibodyAnti-Alpha-Tubulin 1 antibodyAnti-Testis-Specific Alpha-Tubulin antibodyAnti-Tubulin H2-Alpha antibodyAnti-Tubulin Alpha-1 Chain antibodyAnti-TUBA4A antibodyAnti-TUBA1 antibody |
Information sourced from Uniprot.org