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> Anti-MMBP Antibody (5B968)

Anti-MMBP Antibody (5B968)

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Referencia NB-64-117796-100uL

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Anti-MMBP Antibody (5B968)

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Catalog No. TMAC-02448 Copy Product Info
Anti-MMBP Antibody (5B968) is a Rabbit antibody targeting MMBP. Anti-MMBP Antibody (5B968) can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-MMBP Antibody (5B968) is a Rabbit antibody targeting MMBP. Anti-MMBP Antibody (5B968) can be used in WB.
Ig Type
IgG
Clone
5B968
Reactivity
Escherichia coli
Verified Activity
1. Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.
Anti-MMBP Antibody (5B968)
Application
WB
Recommended Dose
WB: 1:1000-5000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundPlasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
P0AEX9
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

MMBPMBPMaltose-binding protein (MBP)Maltose/maltodextrin-binding periplasmic proteinMaltodextrin-binding proteinmalE
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