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> HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein, Human, MHC (His & Avi)

HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein, Human, MHC (His & Avi)

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HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein, Human, MHC (His & Avi)

(Synonyms: sHLA-E, QA1, MHC class I antigen E, MHC, HLAE) Copy Product Info

Synonyms: sHLA-E, QA1, MHC class I antigen E, MHC, HLAE

Catalog No. TMPK-01452 Copy Product Info
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP13747(HLA-E*01:03)&P61769(B2M)&VMAPKTLVL
ConstructionGly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPKTLVL peptide
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymssHLA-E, QA1, MHC class I antigen E, MHC, HLAE
Research Background
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Chemical Properties
Molecular WeightThe protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein, Human, MHC (His & Avi) chemical structure | HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein, Human, MHC (His & Avi) molecular weight