Size : 5g
Telefonnummer : +1 850 650 7790
Synonyms:
| Description | Sudan Black B is a lipophilic diazo dye commonly used in histochemistry to stain neutral lipids, phospholipids, lipoproteins, and lipofuscin within the cytoplasm, facilitating studies of lipid and cellular metabolism. |
| In vitro | Sudan Black B is also applicable to frozen samples, making it suitable for combined use with SA-β-gal in detecting cellular senescence. Sudan Black B is an intensely lipophilic reagent with high affinity for the lipid components of lipofuscin. Under optical microscopy, Sudan Black B-lipofuscin positive reactions appear as blue-black intracellular granules in cells and frozen tissues, while exhibiting brown-black granules in formalin-fixed paraffin-embedded (FFPE) tissues [1]. |
| Molecular Weight | 456.54 |
| Formula | C29H24N6 |
| Cas No. | 4197-25-5 |
| Smiles | N(=NC1=CC=C(N=NC2=CC=C3NC(NC4=CC=CC2=C43)(C)C)C=5C=CC=CC15)C=6C=CC=CC6 |
| Storage | Keep away from direct sunlight Store at -20°C Shipping with blue ice/Shipping at ambient temperature. | ||||||||||
| Solubility Information | H2O: < 1 mg/mL (insoluble) DMSO: 1 mg/mL (2.19 mM), Sonication is recommended. | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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