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> Goat IL-12p40(Interleukin 12 p40) ELISA Kit

Goat IL-12p40(Interleukin 12 p40) ELISA Kit

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Katalog-Nummer NB-66-26907-48T

Size : 48T

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Goat IL-12p40(Interleukin 12 p40) ELISA Kit
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ELK5870
Size： ：
48 T
96 T
96 T*5
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Overview

Product name: Goat IL-12p40(Interleukin 12 p40) ELISA Kit
Reactivity: Goat
Alternative Names: IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Infection immunity;Immune molecule;
Uniprot ID: P68221
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.996 1.908
500.00 1.593 1.505
250.00 1.197 1.109
125.00 0.872 0.784
62.50 0.516 0.428
31.25 0.315 0.227
15.63 0.215 0.127
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-12p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12p40 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 81-93% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 87-98% 85-92% 79-96%
EDTA plasma(n=5) 88-97% 90-99% 87-103% 82-97%
Heparin plasma(n=5) 85-94% 90-99% 82-101% 91-102%