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> Beta-Catenin Protein, Mouse, Recombinant (His & GST)

Beta-Catenin Protein, Mouse, Recombinant (His & GST)

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Beta-Catenin Protein, Mouse, Recombinant (His & GST)

(Synonyms: β-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc) Copy Product Info

Synonyms: β-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc

Catalog No. TMPY-02985 Copy Product Info
Beta-Catenin Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is Q02248.
Other Forms of Beta-Catenin Protein, Mouse, Recombinant (His & GST):
Beta-Catenin Protein, Human, Recombinant (His & GST)TMPY-02205
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For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Beta-Catenin Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is Q02248.
Species
Mouse
Expression System
Baculovirus Insect Cells
TagHis, GST
Accession NumberQ02248
ConstructionA DNA sequence encoding the mouse CTNNB1 (Q02248) (Met1-Leu781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 80 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsβ-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc
Research Background
beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Chemical Properties
Molecular Weight113 kDa (predicted); 115 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

catenin β 1catenin b 1
Related Tags: Beta-Catenin Protein, Mouse, Recombinant (His & GST) chemical structure | Beta-Catenin Protein, Mouse, Recombinant (His & GST) molecular weight