Enzymes for plant culture
Protoplasts are spherical naked plant cells produced by the removal of the cell wall with digestive enzymes. Plant protoplasts can be cultured in defined media and will form a new cell wall, divide, and in many cases regenerate complete plants. Protoplasts provide a unique experimental system for studies of the structure and function of plant cells.
Enzymatic method is a very widely used technique for the isolation of protoplasts. The advantages of enzymatic method include good yield of viable cells, and minimal or no damage to the protoplasts. Protoplasts can be isolated from a wide variety of tissues and organs that include leaves, roots, shoot apices, fruits, embryos and microspores.
The enzymatic isolation of protoplasts can be carried out by two approaches:
Two step or sequential method:
The tissue is first treated with pectinase (macerozyme) to separate cells by degrading middle lamella. These free cells are then exposed to cellulose to release protoplasts. Pectinase breaks up the cell aggregates into individual cells while cellulose removes the cell wall proper.
One step or simultaneous method:
This is the preferred method for protoplast isolation. It involves the simultaneous use of both the enzymes — macerozyme and cellulose.
Frequently Asked Questions (FAQ)
Where can I find enzymes products on the Neo Biotech website?
enzymes products are available in this section and can be sorted by product families or sub-categories, such as Macerozyme. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the enzymes category?
The enzymes category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Macerozyme sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a enzymes product?
To find a enzymes product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Neo Biotech stock several product codes in the enzymes category?
Yes, Neo Biotech distributes several product references in the enzymes category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for enzymes products?
Yes, you can request a quote for enzymes products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a enzymes product is available to order?
The availability of a enzymes product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.
What should I do if I cannot find the enzymes product I am looking for?
If the enzymes product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all enzymes products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.