Size : 100mg
Phone : +1 850 650 7790
Synonyms: NSC 527017
| Description | Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity. |
| Targets & IC50 | Adherence to plastic:5.1 mg/L (PMNs), Leishmania donovani:0.06 µg/mL, Trichophyton mentagrophytes:0.02 µg/mL, Candida species:2-8 µg/mL (MIC50) |
| In vitro | METHODS: Human normal colon epithelial cells CCD 841 CoTr and human colorectal cancer cells HT-29 were treated with Amphotericin B (0.05-25 µg/mL) for 24 h. Cell viability was measured by Neutral Red method. RESULTS: Higher concentrations of Amphotericin B were toxic to CCD 841 CoTr and HT-29 cells, with IC50 values of 8.7 µg/mL and 21.2 µg/mL, respectively. [1] METHODS: Candida albicans were treated with Amphotericin B (100 µM) for 4-16 min and imaged using the FLIM technique. RESULTS: Amphotericin B preferentially binds to the cell wall and does not efficiently cross the barrier covering the cell membrane.Amphotericin B can more readily pass through the cell wall barrier of young cells during the emergence stage. [2] |
| In vivo | METHODS: To assay antifungal activity in vivo, Amphotericin B (0.25-4 mg/kg) was administered as a single intraperitoneal injection to C. albicans K-1-infected ICR/Swiss mice. RESULTS: Only the highest single dose of Amphotericin B treatment significantly reduced the number of colonies compared to the number of colonies at the start of treatment. [3] |
| Synonyms | NSC 527017 |
| Kinase Assay | THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer. |
| Cell Research | Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer. |
| Molecular Weight | 924.08 |
| Formula | C47H73NO17 |
| Cas No. | 1397-89-3 |
| Smiles | 22[C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(O)=O)O2 |
| Relative Density. | 1.34 g/cm3 |
| Storage | Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
| Solubility Information | H2O: < 1 mg/mL (insoluble or slightly soluble) DMSO: 51 mg/mL (55.19 mM), Sonication is recommended.  Ethanol: < 1 mg/mL (insoluble or slightly soluble) | ||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (5.41 mM), Suspension. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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